TSH Receptor (TSHR) Mouse Monoclonal Antibody [Clone ID: 4C1]

CAT#: SM1348P

TSH Receptor (TSHR) mouse monoclonal antibody, clone 4C1, Purified

Product Images

TSHR antibody staining of Paraffin Embedded Human thyroid.

Specifications

Product Data

• Clone Name: 4C1
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/100-1/1,000 diluted antibody to label 10e6 cells in 100 µl.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 1/1,000-1/2,000 (does not require protein digestion pre-treatment nor antigen retrieval using heat treatment prior to staining of paraffin sections).
  Histology positive control: Thyroid gland.
  Immunoprecipitation.
  Western blotting: 1/1,000.
  According to customer information, this antibody has been used successfully on a peptide microarray (2).
  Not Suitable for ELISA.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: External domain of Human TSH receptor
• Specificity: Recognizes the Human thyroid stimulating hormone (TSH) receptor binding to an extracellular domain.
  The epitope has been mapped to aa 379-384 (2).
• Formulation: PBS containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: thyroid stimulating hormone receptor
• Database Link:
  Entrez Gene 7253 Human
  UniProt ID P16473
• Background: Thyrotropin Receptor, a Glycoprotein Hormone Receptor, plays a central role in controlling thyroid cell metabolism. The TSH receptor can interact with both TSH and autoantibodies against the TSH receptor (thyroid-stimulating immunoglobulins, TSI). Mutations in the receptor are the cause for hyperthyroidism such as gestational hyperthyroidism (hypersensitivity to chorionic gonadotropin) and Grave's disease (autoimmune hyperthyroidism). Multiple isoforms of the thyrotropin receptor are produced by altervative splicing.
  The thyrotropin receptor has been reported in adipose, adrenal, brain, eye, heart, kidney, skin, thymus, and thyroid. ESTs have been isolated from brain, placenta, and thyroid libraries.
• Synonyms: Thyroid-stimulating hormone receptor, TSH-receptor, TSH-R, LGR3