HLA Class II DP, DR, DQ Mouse Monoclonal Antibody [Clone ID: WR18]

CAT#: SM1225F

HLA Class II DP, DR, DQ mouse monoclonal antibody, clone WR18, FITC

Specifications

Product Data

• Clone Name: WR18
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10e6 cells or 100 µl whole blood.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human HLA Class II.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS.0 myeloma cell line.
• Specificity: This antibody recognises a common determinant on the beta chain of Human MHC Class II, and stains cells expressing HLA-DR, DP and DQ.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilzer.
  Label: FITC
  State: Liquid purified IgG fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: The major histocompatibility complex (MHC) is a large genomic region or gene family found in most vertebrates containing many genes with important immune system roles. In humans, the MHC spans almost 4 megabases of chromosome 6 and includes more than 200 known genes, of which about half have known immmunological functions. The best known genes in the MHC region are the subset that encodes cell-surface antigen-presenting proteins. In humans, these genes are referred to as human leukocyte antigen (HLA) genes. The most intensely studied HLA genes are: HLA A, HLA B, HLA C, HLA DPA, HLA DPB1, HLA DQA1, HLA DQB1, HLA DRA, and HLA DRB1. In humans, the HLA is divided into three regions: Class I, II, and III. The A, B, and C genes belong to HLA class I while the six D genes belong to class II.
  The antigen is expressed by B lymphocytes, monocytes and activated T lymphocytes.
• Synonyms: MHC class II antigen DP, DR, DQ, HLA class II histocompatibility antigen DP, DR, DQ