HLA Class I ABC Mouse Monoclonal Antibody [Clone ID: W6/32]
HLA Class I ABC mouse monoclonal antibody, clone W6/32, Low Endotoxin
|Applications||ELISA, FC, FN, IF, IHC, IP|
Immunoprecipitation (Ref 1).
Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl. This product is routinely tested in Flow Cytometry on Human peripheral blood lymphocytes.
Immunohistochemistry on Frozen Sections (See Ref 6).
Positive Control Tissue: Tonsil.
Not suitable for Immunohistochemistry on Paraffin Sections.
|Reactivity||Bovine, Chimpanzee, Feline, Human, Macaque, Monkey, Primate, Baboon|
|Immunogen||Purified Human tonsil lymphocyte membranes.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line
|Specificity||SM1222LE recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha-3 and alpha-2 domains (Ref 7). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells.|
|Formulation||PBS without preservatives or stabilizers.
Endotoxin level: Less than 0.01 EU/µg
State: Low Endotoxin
State: Liquid purified IgG fraction
|Purification||Affinity Chromatography on Protein G|
|Storage||Store the antibody undiluted at -20°C only.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch|
|Synonyms||MHC class I ABC, HLA Class 2 ABC|
|The use of this Antibodies has been cited in the following citations:|
Fibromodulin as a novel tumor-associated antigen (TAA) in chronic lymphocytic leukemia (CLL), which allows expansion of specific CD8+ autologous T lymphocytes
,Mayr C, Bund D, Schlee M, Moosmann A, Kofler DM, Hallek M, Wendtner CM,
,PubMed ID 15471955
Transduction of CLL cells by CD40 ligand enhances an antigen-specific immune recognition by autologous T cells
,Mayr C, Kofler DM, B|ning H, Bund D, Hallek M, Wendtner CM,
,PubMed ID 16014560
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