HLA Class I ABC Mouse Monoclonal Antibody [Clone ID: W6/32]

CAT#: SM1222LE

HLA Class I ABC mouse monoclonal antibody, clone W6/32, Low Endotoxin

Product Images

Staining of peripheral blood lymphocytes with anti-HLA-ABC - Low Endotoxin (SM1222LE)

Specifications

Product Data

• Clone Name: W6/32
• Applications: ELISA, FC, FN, IF, IHC, IP
• Recommended Dilution: ELISA.
  Immunoprecipitation (Ref 1).
  Functional Assays.
  Immunofluoresecnce.
  Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10⁶ cells in 100 µl. This product is routinely tested in Flow Cytometry on Human peripheral blood lymphocytes.
  Immunohistochemistry on Frozen Sections (See Ref 6).
  Positive Control Tissue: Tonsil.
  Not suitable for Immunohistochemistry on Paraffin Sections.
• Reactivities: Bovine, Chimpanzee, Feline, Human, Macaque, Monkey, Primate, Baboon
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified Human tonsil lymphocyte membranes.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line
• Specificity: SM1222LE recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha-3 and alpha-2 domains (Ref 7). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells.
• Formulation: PBS without preservatives or stabilizers.
  Endotoxin level: Less than 0.01 EU/µg
  State: Low Endotoxin
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at -20°C only.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch
• Synonyms: MHC class I ABC, HLA Class 2 ABC