B7-2 (CD86) Mouse Monoclonal Antibody [Clone ID: BU63]

CAT#: SM1167PP

B7-2 (CD86) mouse monoclonal antibody, clone BU63, PerCP

Specifications

Product Data

• Clone Name: BU63
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (1 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: B-lymphoblastoid cell line ARH 77
• Specificity: This antibody reacts with CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
• Formulation: PBS
  Label: PerCP
  State: Liquid purified Ig fraction
  Stabilizer: 0.2% (w/v) high-grade protease free BSA
  Preservative: 15mM Sodium Azide
  Label: Conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.
  The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: PerCP
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  This products is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD86 molecule
• Database Link:
  Entrez Gene 942 Human
  UniProt ID P42081
• Background: CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
• Synonyms: CD28LG2, Activation B7-2 antigen, B70, BU63, B7.2, FUN-1