CD45 (PTPRC) (CD45RO) Mouse Monoclonal Antibody [Clone ID: UCHL1]

CAT#: SM1117S

CD45 (PTPRC) (CD45RO) mouse monoclonal antibody, clone UCHL1, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human lymph node stained with CD45RO antibody Cat.-No. SM1117S using peroxidase conjugate and DAB chromogen. Note cell membrane staining of T lymphocytes.

Specifications

Product Data

• Clone Name: UCHL1
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/100
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Recommended Positive Control: Tonsil or lymph node.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Interleukin-2-dependent Human T lymphocytes.
• Specificity: UCHL-1 reacts with mature activated T cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. Shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. It rarely stains B cell lymphomas.
  Cellular Localization: Membranous.
• Formulation: State: Supernatant
  State: Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein tyrosine phosphatase, receptor type C
• Database Link:
  Entrez Gene 9332 HumanEntrez Gene 5788 Human
  UniProt ID P08575
• Background: CD45RO is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA). It expressed on most thymocytes, monocytes, macrophages and granulocytes. It is not expressed on normal B lymphocytes. Intrathymic T cell maturation progresses from CD45RA-/CD45RO+ to CD45RA+/CD45RO-; while peripheral T cell maturation progresses from CD45RA+/CD45RO- to CD45RA-/CD45RO+.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane