CD33 Mouse Monoclonal Antibody [Clone ID: WM53]

CAT#: SM1103PS

CD33 mouse monoclonal antibody, clone WM53, Purified

Product Images

Staining of human peripheral blood monocytes with Mouse anti Human CD33 antibody.

Specifications

Product Data

• Clone Name: WM53
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/10-1/50 diluted antibody to label 10e6 cells.
  Western blot.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections: 1/50-1/100 (Overnight incubation recommended).
  The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.
  Positive Control Tissue: Lymphatic tissue.
  Not suitable on Paraffin Embedded Sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human AML cells.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: Recognizes the CD33 cell surface glycoprotein, a 67kD molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD33 molecule
• Database Link:
  Entrez Gene 945 Human
  UniProt ID P20138
• Background: CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.
• Synonyms: Siglec-3, gp67