CD40 Mouse Monoclonal Antibody [Clone ID: LOB7/6]

CAT#: SM1102PT

CD40 mouse monoclonal antibody, clone LOB7/6, Purified

Product Images

Immunofluorescence staining of a human tonsil cryostetion with Mouse anti Human CD40 antibody, clone LOB7/6, red in A and Mouse anti Human CD21 antibody, clone LB21, green in B. The merged image is C with nuclei counterstained blue using DAPI. Medium power.

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  Immunofluorescence staining of a human tonsil cryostetion with Mouse anti Human CD40 antibody, clone LOB7/6, red in A and Mouse anti Human CD21 antibody, clone LB21, green in B. The merged image is C with nuclei counterstained blue using DAPI. High power.

Specifications

Product Data

• Clone Name: LOB7/6
• Applications: FC, IF, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 10 µl of 20 µg/ml diluted antibody to label 10⁶ cells in 100 µl.
  Immunohistochemistry on Frozen sections: 1-10 mg/ml.
  Immunohistochemistry on Paraffin sections: 1-10 µg/ml. This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining eg. pronase or trypsin.
  Recommended Positive Control: Human Tonsil.
  Immunoprecipitation.
• Reactivities: Canine, Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: CD40 Ig(Fc) fusion protein containing the EC region of human CD40 and Fc region of Human IgG.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody Clone LOB7/6 recognizes the CD40 cell surface antigen.
• Formulation: Tris buffered saline containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 958 Human
  UniProt ID P25942
• Background: CD40 is a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centres, and is vital in T cell-B cell interactions.
• Synonyms: TNFRSF5, CDw40, Bp50