CD35 (CR1) Mouse Monoclonal Antibody [Clone ID: E11]

CAT#: SM1097P

CD35 (CR1) mouse monoclonal antibody, clone E11, Purified

Size: 100 ug 200 ug

Conjugation: Unconjugated FITC


USD 556.00

5 Days*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name E11
Applications FC, IHC, WB
Recommended Dilution Flow Cytometry: Use 10 µl of 1/50-1/200 diluted antibody to label 106 cells or 100 µl whole blood.
Immunohistochemistry on Frozen Section: 1-10 µg/ml.
Immunohistochemistry on Paraffin Section: 1-10 µg/ml.
Requires antigen retrieval using heat treatment prior to staining of Paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Recommended Positive ControlHuman Tonsil.
Western Blotting (Ref.3).
Reactivities Human, Monkey, Baboon
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human acute monocytic leukaemia cells.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity This antibody is specific for CD35, a single chain cell surface glycoprotein which exists in four allotypic forms (A,B,C,D) of 190kD, 220kD, 160kD and 250kD respectively.
Formulation PBS
State: Purified
State: Liquid purified IgG fraction from Tissue Culture Supernatant
Preservative: 0.09% Sodium Azide
Concentration lot specific
Purification Affinity Chromatography on Protein A
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name complement component 3b/4b receptor 1 (Knops blood group)
Background CD35 is a single chain cell surface glycoprotein which exists in four allotypic forms (A,B,C,D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement.
Synonyms Complement receptor type 1, CR1, C3b/C4b receptor
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.