CD14 Mouse Monoclonal Antibody [Clone ID: Tük4]

CAT#: SM1066RT

CD14 mouse monoclonal antibody, clone Tük4, PE

Product Images

Staining of human peripheral blood monocytes probed with Mouse anti Human CD14 - RPE

Specifications

Product Data

• Clone Name: Tük4
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10⁶ cells or 100 µl whole blood.
• Reactivities: Bovine, Canine, Feline, Goat, Human, Mink, Monkey, Porcine, Rabbit, Sheep
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: CD14 Human
• Specificity: This antibody recognises the CD14 cell surface antigen, expressed strongly by most peripheral blood monocytes and weakly on granulocytes.
  Clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose-dependent manner. The use of SM1066LE is recommended for this purpose.
• Formulation: PBS
  Label: PE
  State: Lyophilized purified IgG fraction
  Stabilizer: 1% BSA, 5% Sucrose
  Preservative: 0.09% Sodium Azide
  Label: R. Phycoerythrin
• Reconstitution Method: Restore with 1 ml distilled water
• Purification: Affinity Chromatography on Protein G
• Conjugation: PE
• Storage: Prior to and following reconstitution store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD14 molecule
• Database Link:
  Entrez Gene 929 Human
  UniProt ID P08571
• Background: CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.
• Synonyms: CD14