Thy1 Mouse Monoclonal Antibody [Clone ID: OX-7]
Specifications
| Product Data | |
| Clone Name | OX-7 |
|---|---|
| Application | FC, IHC, IP, WB |
| Recommended Dilution | Western Blot. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Flow Cytometry: Use 10 µl of 1/50-1/100 dilted antibody to label 10e6 cells in 100 µl. This product is routinely tested in flow cytometry on Rat thymocytes. |
| Reactivity | Guinea Pig, Mouse, Rabbit, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Rat Thy 1 antigen. |
| Specificity | This antibody recognises Rat and Mouse CD90, also known as Thy1.1, expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (Mouse), immature B cells (rat) and connective tissues. Since Thy1.1 is a monomorphic determinant in Rat but polymorphic in Mice, clone MRC OX-7 reacts with Thy1.1 Mice e.g. AKR and FVB Mice, but not Thy1.2 Mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using fluorescence microscopy. (5) This clone has been reported to induce glomerular nephritis in Wistar Rats. (8) Affinity of the Fab' of MRC OX-7: Rat Thy1: 3 x 10e9me1 Mouse Thy1.1: 3 x 10e8m-1 (1). |
| Buffer | PBS, pH7.2 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction. |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein G. |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | thymus cell antigen 1, theta |
| Database Link | |
| Background | Thy1.1 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues. |
| Synonyms | Thy-1, THY1, CDw90 |
| Reference Data | |
| Protein Categories | CD Markers, Intracellular Proteins, Membrane Proteins |
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