CD11b Rat Monoclonal Antibody [Clone ID: M1/70.15]

CAT#: SM014R

CD11b rat monoclonal antibody, clone M1/70.15, PE


USD 579.00

5 Days*

Size
    • 100 Tests

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Specifications

Product Data
Clone Name M1/70.15
Applications FC
Recommended Dilution Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Reactivities Human, Mouse, Rabbit
Host Rat
Isotype IgG2b
Clonality Monoclonal
Immunogen T cell enriched splenocytes from B10 mice.
Spleen cells from an immunised DA rat were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line.
Specificity This antibody recognises the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1).
Clone M1/70.15 has been reported to block iC3b binding to its receptor. SM014A is recommended for this purpose.
Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue.
Formulation PBS, pH 7.4 containing 0.09% Sodium Azide, 1% BSA, 5% Sucrose
Label: PE
State: Lyophilized purified IgG fraction.
Label: R. Phycoerythrin (RPE)
Reconstitution Method Restore with distilled water.
Purification Affinity Chromatography on Protein G
Conjugation PE
Storage Prior to and following reconstitution store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Stability Shelf life: one year from despatch.
Background CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages.
CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
Synonyms ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.