CD11b Rat Monoclonal Antibody [Clone ID: M1/70.15]

CAT#: SM014PS

CD11b rat monoclonal antibody, clone M1/70.15, Purified

Product Images

Staining of Mouse peritoneal Macrophages with Rat anti Mouse CD11b Antibody

Specifications

Product Data

• Clone Name: M1/70.15
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Western blot.
  Flow Cytometry: 1/50-1/200.
  This product is routinely tested on Mouse peritoneal Macrophages.
  Immunoprecipitation.
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. (4)
• Reactivities: Human, Mouse, Rabbit
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: T cell enriched splenocytes from B10 mice.
  Spleen cells from immunised DA Rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
• Specificity: This antibody recognises the Murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1).
  Clone M1/70.15 has been reported to block iC3b binding to its receptor. SM014A is recommended for this purpose.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from tissue culture supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P05555
• Background: MAC-1 is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1