CD11b Rat Monoclonal Antibody [Clone ID: M1/70.15]

CAT#: SM014LE

CD11b rat monoclonal antibody, clone M1/70.15, Low Endotoxin

Specifications

Product Data

• Clone Name: M1/70.15
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections.
  Immunoprecipitation.
  Flow Cytometry: 1/50-1/100 (routinely tested on Mouse peritoneal macrophages).
  Functional Assays: Clone M1/70.15 has been reported to block iC3b binding to its receptor.
  Immunofluorescence.
• Reactivities: Human, Mouse, Rabbit
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: T cell enriched splenocytes from B10 mice.
  Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
• Specificity: This antibody recognises the CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.
• Formulation: State: Low Endotoxin
  State: Liquid purified Ig fraction without preservatives.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at -20°C only.
  Storage in frost free freezers is not recommended.
  Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P05555
• Background: The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
• Note: Endotoxin Level: < 0.01EU/µg