CD11b Rat Monoclonal Antibody [Clone ID: M1/70.15]

CAT#: SM014F

CD11b rat monoclonal antibody, clone M1/70.15, FITC

Product Images

Staining of mouse peritoneal macrophages cells with RAT ANTI MOUSE CD11b:FITC

Specifications

Product Data

• Clone Name: M1/70.15
• Applications: FC
• Recommended Dilution: Flow cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl; The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Human, Mouse, Rabbit
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA rats were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line.
• Specificity: This antibody recognises the CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1). Clone M1/70.15 has been reported to block iC3b binding to its receptor. SM014A is recommended for this purpose. Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P05555
• Background: MAC-1 is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1