CD11b Rat Monoclonal Antibody [Clone ID: M1/70.15]

CAT#: SM014BT

CD11b rat monoclonal antibody, clone M1/70.15, Biotin

Specifications

Product Data

• Clone Name: M1/70.15
• Applications: FC
• Recommended Dilution: Flow cytometry: Neat.
  Positive control: mouse peritoneal macrophages.
• Reactivities: Human, Mouse, Rabbit
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: T cell enriched splenocytes from B10 mice
• Specificity: This antibody recognises the CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.
  Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue (4).
• Formulation: 0.09% Sodium Azide, 1% Bovine Serum Albumin
  Label: Biotin
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P05555
• Background: The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1