Cd8a Rat Monoclonal Antibody [Clone ID: KT15]

CAT#: SM011PS

Cd8a rat monoclonal antibody, clone KT15, Purified

Product Images

Staining of Mouse spleen cells with Rat anti Mouse CD8 antibody.

Specifications

Product Data

• Clone Name: KT15
• Applications: FC, IF, IHC
• Recommended Dilution: Immunofluorescence.
  Flow Cytometry: Use 10 µl of 1/100-1/200 diluted antibody to label 10e6 cells in 100 µl.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: T cell clone, C6.
• Specificity: This antibody recognizes Ly2.1 and Ly2.2 cells; reacting with the CD8 alpha chain (non polymorphic). It is weakly cytotoxic.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD8 antigen, alpha chain
• Database Link:
  Entrez Gene 12525 Mouse
  UniProt ID P01731
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL