Biotin Goat Polyclonal Antibody

CAT#: R1615P

Biotin goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, ID, IHC, IP, WB
• Recommended Dilution: Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/20,000.
  Western blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/1,000-1/5,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Biotin coupled to sepharose beads.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
• Formulation: 0.02 Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid (sterile filtered) purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store vial at 2-8°C prior to opening.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
  Avoid cycles of freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
• Synonyms: Vitamin B7, Vitamin H