Alanine aminotransferase 1 / ALT1 Sheep Polyclonal Antibody

CAT#: R1550PS

Alanine aminotransferase 1 / ALT1 sheep polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot: 1/500-1/3000.
  ELISA: 1/5000-1/25000.
  This antibody has been assayed against 1.0 µg of Alanine transaminase [pig heart] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-sheep IgG [H&L] (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/8,000 of the reconstitution concentration is suggested.
• Reactivities: Porcine
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Alanine transaminase (ALT) from pig heart
• Specificity: This antibody detects porcine alanine transaminase. Cross reactivity against alanine transaminase from other sources is unknown.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum as well as purified and partially purified alanine transaminase [pig heart].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  State: Purified
  State: Lyophilized purified Ig fraction
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P13191
• Background: Alanine transaminase (ALT) is also referred to as Glutamic pyruvic transaminase (GPT).
• Synonyms: GPT, AAT1, GPT1
• Note: Conjugates available. Please ask for details.