Os04g0627000 (C-term) Rabbit Polyclonal Antibody
Os04g0627000 (C-term) rabbit polyclonal antibody, Serum
|Applications||Assay, ELISA, IP, WB|
|Recommended Dilution||This antibody reacts with human ROC2 by Western blot (1:500-1:1,000) and Immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins does occur. A 12.6 kDa band corresponding to human ROC2 is detected.
Most cell lines expressing ROC2 can be used as a positive control.
|Reactivity||C. elegans, Human, Mouse, Zebrafish|
|Immunogen||Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 102-113 of Human ROC2 (C-terminal) coupled to KLH.|
|Specificity||This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
This product reacts with human, mouse, C.elgans and zebra fish ROC2. Cross reactivity may also occur with ROC2 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and APC11.
State: Liquid (sterile filtered) with 0.01% (w/v) Sodium Azide as preservative.
|Purification||Delipidation and defibrination.|
|Storage||Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||ROC1 also known as RING-box protein 2, Rbx2, RING finger protein 7, Regulator of cullins 2, CKII betabinding protein 1, and CKBBP1, is a probable component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC2 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. ROC2 may play a role in protecting cells from apoptosis induced by redox agents. ROC2 has a cytoplasmic and nuclear localization and is expressed in heart, liver, skeletal muscle and pancreas tissues, and at very low levels in brain, placenta and lung. 1,10-phenanthroline induces ROC2 expression. The RING-type zinc finger domain is essential for ubiquitin ligase activity. Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.|
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