ROC1 (RBX1) (C-term) Rabbit Polyclonal Antibody
ROC1 (RBX1) (C-term) rabbit polyclonal antibody, Serum
|Applications||Assay, ELISA, IP, WB|
|Recommended Dilution||This antibody reacts with human ROC1 by Western blot (1:500-1:1,000) and Immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related cullin proteins does occur. Coimmunoprecipitation of ubiquitin ligase activity will also occur. A 12.2 kDa band corresponding to human ROC1 is detected. Most cell lines expressing ROC1 can be used as a positive control.|
|Reactivity||C. elegans, Human, Mouse, Zebrafish|
|Immunogen||Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 94-108 of Human ROC1 (C-terminal) coupled to KLH.|
|Specificity||This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human, mouse, C.elgans and zebra fish ROC1. Cross reactivity may also occur with ROC1 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC2 and APC11.|
State: Liquid (sterile filtered) with 0.01% (w/v) Sodium Azide as preservative.
|Concentration||85 mg/ml (by Refractometry)|
|Purification||Delipidation and defibrination.|
|Storage||Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||ROC1 also known as RING-box protein 1, Rbx1, Regulator of cullins 1, RING finger protein 75, and ZYP protein, is a component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC1 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. The RING-type zinc finger domain is essential for ubiquitin ligase activity. ROC1 probably also stimulates CDC34 autoubiquitination and promotes the neddylation of CUL1 and probably CUL2. ROC1 has a cytoplasmic and nuclear localization and is widely expressed in most tissues.|
|Synonyms||Protein ZYP, ROC1|