AKT2 Rabbit Polyclonal Antibody

CAT#: R1467

AKT2 rabbit polyclonal antibody, Aff - Purified


USD 662.00

3 Days*

Size
    • 100 ug

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Specifications

Product Data
Applications WB
Recommended Dilution This antibody was tested by ELISA and Immunoblotting and was found to be reactive with both unphosphorylated and phosphorylated AKT2 in a lysate of HEK293 cells. Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation.
Recommended Dilution(s): This product has been assayed by immunoblot against a HEK293 cell lysate and is reactive at a 1:1,000 dilution showing a band at approximately 60 kDa. A working dilution of 1:4,000 to 1:16,000 is suggested for this product in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) code # TMBE-100 as a substrate for 30 minutes at room temperature against 0.1 ug of the immunizing peptide.
Reactivities Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide of human AKT2 conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity This affinity purified antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 , with 0.01% Sodium Azide as preservative.
State: Aff - Purified
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Immunoaffinity purification.
Conjugation Unconjugated
Storage Store vial at -20°C prior to opening.
Centrifuge product if not completely clear after standing at
room temperature. For extended storage aliquot contents and freeze at -20°C or below.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background AKT2 is also known as V-AKT Murine Thymoma Viral Oncogene Homolog 2 antibody, Rac protein kinase beta antibody, PKB beta antibody or PRKBB antibody. AKT2 is an isoform of the phosphoinositidedependent serine-threonine protein kinase AKT and is enriched in insulin-responsive tissues and has been implicated in the metabolic actions of the hormone. AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2- like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins. AKT2 mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT2 to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT2 dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT2 has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Synonyms RAC-PK-beta, Protein kinase Akt-2, Protein kinase B beta
Reference Data

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