Laminin Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IHC, IP, WB |
|---|---|
| Recommended Dilution | Anti-Laminin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for Western blotting for highly sensitive qualitative analysis. Recommended Dilutions: This product was assayed by immunoblot and found to be reactive against Laminin at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 ug of Laminin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For Immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. |
| Reactivity | Human |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced against a mixture of synthetic peptides corresponding to regions of adherence on laminin. |
| Specificity | This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Laminin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative and 10 mg/ml Bovine Serum Albumin (Ig and Protease free) as stabilizer. Label: Biotin State: Liquid (sterile filtered) purified Ig fraction. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Rabbit IgG molecule. |
| Concentration | lot specific |
| Purification | Immunoaffinity chromatography. |
| Conjugation | Biotin |
| Storage | Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid repeated freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
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