ycjM Goat Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IP, WB |
|---|---|
| Recommended Dilution | Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of Sucrose Phosphorylase E.coli in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,500 to 1/5,000 of the reconstitution concentration is suggested for this product. |
| Reactivity | Escherichia coli |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Sucrose phosphorylase from E.coli |
| Specificity | This antibody detects E.coli Sucrose phosphorylase. Cross reactivity against Sucrose phosphorylase from other sources is unknown. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified Sucrose phosphorylase E.coli. |
| Buffer | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/m BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) |
| Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
| Concentration | lot specific |
| Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
| Conjugation | HRP |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Database Link | |
| Synonyms | Sucrose glucosyltransferase (E. coli) |
| Reference Data | |
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