Penicillinase Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IP, WB |
|---|---|
| Recommended Dilution | ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of Penicillinase E.clocae in a standard ELISA using peroxidase conjugated affinity purified anti-Rabbit IgG L - Cat# H (Goat) code R1364HRP and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/7,000 to 1/21,000 of the reconstitution concentration is suggested. Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. |
| Reactivity | Enterobacter |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Penicillinase from Enterobacter cloacae |
| Specificity | This antibody detects Penicillinase E. clocae. Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determinedImmunoelectrophoresis give a single precipitin arc against anti-rabbit serum as well as purified and partially purified Penicillinase E. clocae. |
| Buffer | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 State: Ig Fraction State: Lyophilized purified Ig fraction Preservative: 0.01% (w/v) Sodium azide |
| Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
| Concentration | lot specific |
| Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
| Conjugation | Unconjugated |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Reference Data | |
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