Pancreatic Islet Lysate Dog Polyclonal Antibody
$950.00
3 Days*
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | Suitable for Immunoblotting (Western or Dot blot) and ELISA. Recommended Dilutions: This product has been assayed against 1.0 ug of Pancreatic Islet Lysate Porcine Pancreas in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Dog IgG L - Cat# H (Goat) and o-Phenylenediamine (OPD) as a substrate. A working dilution of 1:50 to 1:100 is suggested for this product. This product has been assayed against 1.5 ug of Pancreatic Islet Lysate Porcine Pancreas per lane by Immunoblot (Western) under reducing conditions of SDS-PAGE prior to transfer to nitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG L - Cat# H (Goat) and Diaminobenzidine (DAB) as a substrate. A working dilution of 1:50 to 1:200 is suggested for this product. |
| Reactivity | Porcine |
| Antibody Host | Dog |
| Clonality | Polyclonal |
| Immunogen | Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells. |
| Specificity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum. Cross reactivity was determined by immunoblot (western) to be minimal against porcine kidney and porcine liver cell lysates. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. State: Ig Fraction State: Liquid (sterile filtered) purified Ig fraction. |
| Concentration | lot specific |
| Purification | Multi-step process. |
| Conjugation | Unconjugated |
| Storage | Store vial at -70°C prior to opening. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.