LPO Sheep Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IP, WB |
|---|---|
| Recommended Dilution | Western blot: 1/500 - 1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000 - 1/20,000. This product has been assayed against 1.0 µg of Lactoperoxidase bovine milk in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/10,000 of the reconstitution concentration is suggested. |
| Reactivity | Bovine |
| Antibody Host | Sheep |
| Clonality | Polyclonal |
| Immunogen | Lactoperoxidase from bovine milk |
| Specificity | This product detects bovine Lactoperoxidase. Cross reactivity against Lactoperoxidase from other sources is unknown. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified Lactoperoxidase bovine milk. |
| Buffer | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate Label: Horseradish peroxidase |
| Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
| Concentration | lot specific |
| Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
| Conjugation | HRP |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Database Link | |
| Synonyms | LPO, SAPX, Lactoperoxidase, EC=1.11.1.7, Salivary peroxidase, SPO |
| Reference Data | |
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