LDHA Goat Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IF, IP, WB |
|---|---|
| Recommended Dilution | Suitable for use in ELISA (1/4,000-1/20,000) and Western blot (1/500-1/2,000), Immunoprecipitation (1/100). Expect a band approximately 36 kDa in size corresponding to LDH western blotting in the appropriate cell lysate or extract. |
| Reactivity | Rabbit |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Full length lactate dehydrogenase protein isolated from Rabbit muscle. |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Lactate Dehydrogenase Rabbit Muscle. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species. |
| Buffer | 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 State: Serum State: Lyophilized purified Ig fraction Stabilizer: None Preservative: 0.01% Sodium Azide |
| Reconstitution Method | Restore with 2.0 ml of deionized water (or equivalent) |
| Concentration | lot specific |
| Purification | Prepared from monospecific antiserum by delipidation and defibrination |
| Conjugation | Unconjugated |
| Storage | Prior to reconstitution store at 2-8°C. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Database Link | |
| Background | Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization. |
| Synonyms | LDH-A, L-lactate dehydrogenase A chain, LDH-M, PIG19, NY-REN-59 |
| Reference Data | |
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