GOT1 Sheep Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | Western blot: 1/1,000-1/5,000. ELISA: 1/10,000-1/40,000. This antibody has been assayed against 1.0 µg of aspartate transaminase porcine heart in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested. |
| Reactivity | Porcine |
| Antibody Host | Sheep |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Aspartate aminotransferase / GOT1 from porcine heart |
| Specificity | This antibody detects porcine GOT1. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase and anti-sheep serum as well as purified and partially purified aspartate transaminase / GOT1 porcine heart. |
| Buffer | 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase |
| Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
| Concentration | lot specific |
| Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
| Conjugation | HRP |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Database Link | |
| Background | Aspartate aminotransferase (Glutamate oxaloacetate transaminase / GOT1) is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT (with SGPT) levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower. |
| Synonyms | Aspartate aminotransferase, Transaminase A |
| Note | Aspartate aminotransferase (AST) is also referred to as glutamic oxaloacetic transaminase (GOT). |
| Reference Data | |
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