G6PD Goat Polyclonal Antibody

CAT#: R1085

G6PD goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Conjugation and most immunological methods requiring high titer and specificity.
  Recommended Dilutions: This product has been assayed against 1.0 ug of Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
• Reactivities: Leuconostoc
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides].
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides].
  Cross reactivity against Glucose-6-Phosphate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative.
  State: Serum
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Prepared from monospecific antiserum by a delipidation and defibrination.
• Conjugation: Unconjugated
• Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P11411
• Background: Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency; one benefit of having G6PD deficiency is that it confers a resistance to malaria.
• Synonyms: Glucose-6-phosphate 1-dehydrogenase, Glucose-6-P-Dehydrogenase