G6PD Goat Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | Suitable for Immunoblotting (Western or Dot blot), ELISA, Conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 ug of Glucose-6-Phosphate Dehydrogenase Leuconostoc mesenteroides in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG L - Cat# H (Goat) and ABTS (2,2'-azino-bis-3-ethylbenthiozoline-6-sulfonic acid) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. |
| Reactivity | Leuconostoc |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Glucose-6-Phosphate Dehydrogenase Leuconostoc mesenteroides. |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glucose-6-Phosphate Dehydrogenase Leuconostoc mesenteroides. Cross reactivity against Glucose-6-Phosphate Dehydrogenase from other tissues and species may occur but have not been specifically determined. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative. State: Serum State: Lyophilized purified Ig fraction. |
| Reconstitution Method | Restore with 2.0 ml of deionized water (or equivalent). |
| Concentration | lot specific |
| Purification | Prepared from monospecific antiserum by a delipidation and defibrination. |
| Conjugation | Unconjugated |
| Storage | Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Ambient |
| Database Link | |
| Background | Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency; one benefit of having G6PD deficiency is that it confers a resistance to malaria. |
| Synonyms | Glucose-6-phosphate 1-dehydrogenase, Glucose-6-P-Dehydrogenase |
| Reference Data | |
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