gdh Goat Polyclonal Antibody
|Recommended Dilution||Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Glucose Dehydrogenase [Bacillus] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,500 to 1:32,000 of the reconstitution concentration is suggested for this product.
|Immunogen||Glucose Dehydrogenase [Bacillus].|
|Specificity||Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glucose Dehydrogenase [Bacillus].
Cross reactivity against Glucose Dehydrogenase from other sources is unknown.
|Formulation||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative.
State: Lyophilized purified Ig fraction.
|Reconstitution Method||Restore with 2.0 ml of deionized water (or equivalent).|
|Purification||Prepared from monospecific antiserum by a delipidation and defibrination.|
|Storage||Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below.
This product is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
|Stability||Shelf life: One year from despatch.|
|Background||Glucose Dehydrogenase catalyses the oxidation of D glucose without prior phosphorylation to D beta gluconolactone using NAD or NADP as a coenzyme. The enzyme is a tetrameric protein, each of the 4 identical subunits containing 262 amino acid residues. This family is a subset of a more general family of short chain dehydrogenases and reductases.|
|Synonyms||Glucose 1-dehydrogenase, gdh, BSU03930|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.