CPB1 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | Western blot: 1/250-1/1,000 ELISA: 1/1,000-1/5,000 This product has been assayed against 1.0 µg of Carboxypeptidase B porcine pancreas in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000-1/3,000 of the reconstitution concentration is suggested. |
| Reactivity | Porcine |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Carboxypeptidase B from porcine pancreas |
| Specificity | This product detects porcine Carboxypeptidase B. Cross reactivity against Carboxypeptidase B from other tissues and species is unknown. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Carboxypeptidase B porcine pancreas. |
| Buffer | 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2 Label: HRP State: Lyophilized purified IgG fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate Label: Horseradish peroxidase |
| Reconstitution Method | Restore with 0.1 ml deionized water (or equivalent). |
| Concentration | lot specific |
| Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
| Conjugation | HRP |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Database Link | |
| Background | Carboxypeptidase catalyzes the hydrolysis of the basic amino acids lysine, arginine and ornithine from the C terminal position in polypeptides. It occurs as a zymogen in the pancreas in most vertebrates. |
| Synonyms | CPB1, CPB, PCPB, Pancreas-specific protein, PASP |
| Reference Data | |
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