Bilirubin oxidase Goat Polyclonal Antibody
Bilirubin oxidase goat polyclonal antibody, HRP, Purified
|Applications||ELISA, IP, WB|
|Recommended Dilution||Western blot: 1/500-1/2,500.
This product has been assayed against 1.0 µg of Bilirubin oxidase [Myrothecium verrucaria] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested.
|Immunogen||Bilirubin oxidase from Myrothecium verrucaria|
|Specificity||This antibody detects Bilirubin oxidase [Myrothecium verrucaria]. Cross reactivity against Bilirubin oxidase from other sources is unknown.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anit-peroxidase, anti-goat serum as well as purified and partially purified Bilirubin oxidase [Myrothecium verrucaria].
|Formulation||0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
State: Lyophilized purified Ig fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
Label: Horseradish peroxidase
|Reconstitution Method||Restore with 0.1 ml of deionized water (or equivalent).|
|Concentration||1.0 mg/ml (by UV absorbance at 280 nm)|
|Purification||Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||Bilirubin Oxidase is an enzyme that catalyses the oxidation of bilirubin to biliverdin and other substrates. This enzyme has been expected to be used in the field of clinical pathology for the enzymatic determination
of total bilirubin in serum and the treatment of jaundice and hyperbilirubinemia.