beta 2 Microglobulin (B2M) Rabbit Polyclonal Antibody

SKU R1065HRP

beta 2 Microglobulin (B2M) rabbit polyclonal antibody, HRP

Product Data

• Application: ELISA, EM, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.; Recommended Dilutions:; ELISA: 1/2,000-1/10,000.; Western blot: 1/500-1/2,000.; Immunohistochemistry: 1/200-1/1,000.
• Reactivity: Human
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Beta-2-Microglobulin [Human Urine].
• Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes Delipidation, Salt Fractionation and Ion Exchange Chromatography followed by extensive dialysis against the buffer stated above.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified beta-2-Microglobulin (Human Urine).; Cross reactivity against beta-2-Microglobulin from other sources is unknown.
• Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2; Label: HRP; State: Lyophilized purified IgG fraction; Stabilizer: 10 mg/ml BSA (IgG and Protease free); Preservative: 0.01% Gentamicin Sulfate; Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 5.0 mg/mL by UV absorbance at 280 nm
• Purification: Multistep Process
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: beta-2-microglobulin
• Database Link:
  P61769
• Background: Beta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
• Synonyms: B2M, Beta-2 microglobulin, Beta 2 microglobulin

Reference Data