Alpha-amylase Rabbit Polyclonal Antibody

SKU
R1062PS
Alpha-amylase rabbit polyclonal antibody, Purified
  $870.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IP, WB
Recommended Dilution

Western blot: 1/500-1/5,000.
Immunoprecipitation: 1/100.
ELISA: 1/5,000-1/20,000.
This antibody has been assayed against 1.0 µg of Alpha-amylase in a standard ELISA using peroxidase conjugated affinity purified anti-rabbit IgG (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.

Reactivity Bacillus amyloliquefacies
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Alpha-amylase from Bacillus amyloliquefaciens
Specificity This antibody detects Alpha-amylase (Bacillus amyloliquefaciens). Cross reactivity against Alpha-amylase from other tissues and species may occur but have not been specifically determined.
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alpha Amylase from Bacillus amyloliquefaciens.
Buffer 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
State: Purified
State: Liquid purified Ig fraction
Preservative: 0.01% (w/v) Sodium azide
Concentration lot specific
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation Unconjugated
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Database Link
Background Amylase catalyses the hydrolysis of internal 1,4 glucan links in large linear polymers at internal bonds. The enzyme is present in all living organisms with variable enzymatic activity.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.