CXCL16 Rabbit Polyclonal Antibody

CAT#: PP1204B1

CXCL16 rabbit polyclonal antibody, Biotin

Product Images

Direct ELISA using CXCL16 Antibody Cat.-No [PP1204B]

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human CXCL16 by indirect ELISA (using 100 µl/well antibody solution) a concentration of ~1 µg/ml of this antibody is required. This Biotinylated antibody, allows the detection of 0.2-0.4 ng/well of recombinant Human CXCL16.
  Sandwich ELISA: To detect Human CXCL16 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. This Biotinylated antibody, in conjunction with Affinity purified anti-Human CXCL16 antibody (Cat.-No PP1204P1 or PP1204P2) as a Capture antibody, allows the detection of 0.2-0.4 ng/well of recombinant Human CXCL16.
  Western Blot: To detect Human CXCL16 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. The detection limit for recombinant Human CXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human CXCL16 (Cat.-No AR31138PU).
• Specificity: This antibody recognizes Human CXCL16. Other species not tested.
• Formulation: PBS, pH 7.4 without preservatives.
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction.
• Reconstitution Method: Restore in sterile PBS solution containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography.
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 58191 Human
  UniProt ID Q9H2A7
• Background: CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. It is a type I membrane protein (Potential). Also exists as a soluble form. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.
• Synonyms: C-X-C motif chemokine 16, Small-inducible cytokine B16, SR-PSOX, SCYB16, SRPSOX
• Note: Centrifuge vial prior to opening!