Ccl3 Rabbit Polyclonal Antibody

CAT#: PP038P2

Ccl3 rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat MIP1-alpha (100 ng/ml), a concentration of 8.0-10.0 µg/ml of this antibody is required.
  Indirect ELISA: To detect Rat MIP1-alpha by Indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MIP-1 alpha.
  Sandwich ELISA: To detect Rat MIP1-alpha by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This purified antibody, in conjunction with Biotinylated Anti-Rat MIP1-alpha (Cat.-No PP038B1 or PP038B2) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MIP1-alpha.
  Western Blot: To detect Rat MIP1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MIP1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli recombinant Rat MIP-1-alpha (Cat.-No PA043)
• Specificity: Recognizes Rat MIP-1 alpha.
  Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing an immobilized Rat MIP1-alpha Matrix
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: C-C motif chemokine ligand 3
• Database Link:
  Entrez Gene 25542 Rat
  UniProt ID P50229
• Background: Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.
• Synonyms: CCL-3, MIP-1-alpha, C-C motif chemokine 3, MIP1A, Small-inducible cytokine A3, SCYA3, LD78 alpha, G0S19-1 protein
• Note: Centrifuge vial prior to opening!