Ifng Rat Monoclonal Antibody [Clone ID: RMMG-1]

CAT#: PBL22500-1

Ifng rat monoclonal antibody, clone RMMG-1

Specifications

Product Data

• Clone Name: RMMG-1
• Applications: ELISA, FN, IHC, IP, WB
• Recommended Dilution: ELISA, Neutralization and Western blot.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity:

  Neutralizes mouse IFN-gamma; binds to mouse IFN-gamma with high affinity (K_A ≥ 10⁹ M^-1).

  Assay Used to Measure Bioactivity/Specificity: Binding to mouse interferon gamma (mouse IFN-γ) was determined by the method of Scatchard [Scatchard, G. (1949) Ann. N.Y. Acad. Sci. 51, 660-672]. Neutralization was determined by the ability of this antibody to block the activity of Mouse IFN-γ (PBL 12500) in a cytopathic effect assay using L929 cells challenged with EMCV [Rubinstein et al. (1981) J. Vir. 37:755. Staehelin et al. (1981) P.N.A.S. 78(3):1848]. The activity is also measured in a direct binding ELISA to Mouse IFN-γ coated plates.

• Formulation: Phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
  State: Purified
• Purification: A combination of ion exchange, hydrophobic interaction and size exclusion chromatography
• Conjugation: Unconjugated
• Gene Name: interferon gamma
• Database Link:
  Entrez Gene 15978 Mouse
  UniProt ID P01580
• Background: Interferon-gamma is the sole representative of the class II interferons. Originally, it was designated immune interferon and unlike the Type I interferons, it is inactivated by incubation at pH 2. This interferon is produced by T-cells, B-cells, NK cells, NKT cells, monocytes, macrophages and dendritic cells. Interferon-gamma has many activities in addition to direct antiviral protection of cells. It upregulates cell surface class I and class II MHC which increases the likelihood that foreign antigens will be presented to the adaptive immune system. IFN-gamma is a major Th1 cytokine and amplifies Th1 responses through regulation of cell surface markers and its effects on the growth and differentiation of cells. It inhibits the growth of tumor cells and a variety of other cells and has profound effects on leukocyte trafficking.
  The RMMG-1 antibody binds to mouse IFN-gamma with high affinity and neutralizes its activity. As such, it provides a tool to examine the role of IFN-gamma in a number of models. For instance, Siegmund et al. (2004) have used this antibody in a mouse model of inflammatory colitis to confirm in WT mice that IFN-gamma does not play a major role in the disease process.
• Synonyms: IFN-gamma, gamma IFN