Interferon beta (IFNB1) Rat Monoclonal Antibody [Clone ID: RMMB-1]

CAT#: PBL22400-1

Interferon beta (IFNB1) rat monoclonal antibody, clone RMMB-1

Product Images

Figure 1. Representative binding curve of antibody to recombinant Mu-IFN-β (circle) and to 1% BSA/PBS (square) in a Direct Binding ELISA. High-binding polystyrene plates were coated with either 1 μg/ml Mu-IFN-β or 1% BSA/PBS. Thereafter, titrations of the product were added to the wells. Donkey anti-rat IgG conjugated to HRP was used as the detection antibody. Colorimetric detection was performed using 3, 3', 5, 5'-Tetramethylbenzidine (TMB) substrate. The HRP-TMB reaction was stopped using a diluted H2SO4/HCl solution.

Specifications

Product Data

• Clone Name: RMMB-1
• Applications: ELISA, FN
• Recommended Dilution: Direct Binding ELISA.
  Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of mouse interferon beta (Mu-IFN-β) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Mu-IFN-β provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for effective neutralization of Mu-IFN-β.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Mouse Interferon Beta
• Specificity: Rat Monoclonal Antibody against Mouse Interferon Beta.
  Bioactivity: Weakly neutralizes mouse interferon beta, but not recommended for neutralization assays (for this application products 32400-1 or 32401-1 are recommended); binds to mouse interferon beta.
• Formulation: PBS) containing 0.1% bovine serum albumin (BSA)
  State: Purified
• Conjugation: Unconjugated
• Gene Name: Homo sapiens interferon, beta 1, fibroblast (IFNB1)
• Database Link:
  Entrez Gene 15977 Mouse
  UniProt ID P01574
• Synonyms: IFN-beta, IFNB1, IFB, Fibroblast interferon

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway