Thrombospondin 1 (THBS1) Mouse Monoclonal Antibody [Clone ID: A6.1]

CAT#: DM315-05

Thrombospondin 1 (THBS1) mouse monoclonal antibody, clone A6.1, Purified

Product Images

Formalin Fixed Paraffin Embedded Human tonsil stained with Thrombospodin Antibody Cat.-No DM315 (Clone A6.1).

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  Formalin Fixed Paraffin Embedded Human placenta stained with Thrombospodin Antibody Cat.-No DM315 (Clone A6.1).

Specifications

Product Data

• Clone Name: A6.1
• Applications: IF, IHC, WB
• Recommended Dilution: Immunofluorescence.
  Immunoprecipitation.
  Western Blot.
  Immunohistochemistry on Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
  Recommended Positive Control: Tonsil.
• Reactivities: Mammalian
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
• Specificity: This antibody is specific to 450 kDa protein (non-reduced) and 170 to 180 kDa (reduced) form of Thrombospondin.
  Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum.
• Formulation: State: Purified
  State: Liquid purified Ig fraction
  Preservative: Sodium Azide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: thrombospondin 1
• Database Link:
  UniProt ID P07996
• Background: Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
• Synonyms: TSP, TSP1