MCP3 (CCL7) Mouse Monoclonal Antibody [Clone ID: 542]
$915.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | 542 |
|---|---|
| Application | ELISA, WB |
| Recommended Dilution | ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-3 detection in combination with a monoclonal tracer/detection antibody (HRP conjugated, Cat.-No DM2035HRP or Biotin-conjugated Cat.-No DM2035B). Suggested Capture Coating Dose: 3 µg/well. Substrate: TMB. Western Blot: An antibody concentration of 0.5-2 µg/mL will allow visualization of 0.1 µg/lane of Human MCP-3. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human MCP-3 (M.W. 8.5 kDa containing 76 amino acids residues) |
| Specificity | Reactive with natural and recombinant Human MCP-3. Does not show any cross reaction with other Human Cytokines or growth factors tested such as IL1 beta, IL-8, MCAF, SAA and EGF. |
| Buffer | 0.01M PBS, pH 7.2 without preservatives State: Purified State: Lyophilized purified IgG fraction |
| Reconstitution Method | Restore with Double distillated to adjust the final concentration to 1.00 mg/ml |
| Concentration | 0.1 mg/ml (after reconstitution) |
| Purification | Affinity Chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Gene Name | C-C motif chemokine ligand 7 |
| Database Link | |
| Background | Macrophage/monocyte chemotactic protein-3 (MCP-3), like other two closely related chemokines (MCP1 and MCP-2), is chemotactic to monocytes, but not to neutrophils. MCP-3 showed 78% sequence homology with MCP-1 and 58% sequence homology with MCP-2. The primary receptor for MCP-3 is D6. MCP-3 also competes with MCP-1 to bind to CCR10, and competes with Rantes and MIP-1α to bind to CCR1. MCP-3 stimulates macrophage to secrete proteases. A variety of cancer cell lines express MCP-3, suggesting a role in tumor invasion and metastasia. |
| Synonyms | Small-inducible cytokine A7, CCL-7, MCP-3, C-C motif chemokine 7, SCYA6, SCYA7, NC28 |
| Reference Data | |
| Protein Categories | Chemokines, Cytokines, Membrane Proteins, Secreated Proteins |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.