Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: RCK108]

CAT#: DM188P

Cytokeratin 19 (KRT19) mouse monoclonal antibody, clone RCK108, Purified

Product Images

Immunohistochemistry on Frozen Section of Human liver bile duct epithelium

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  Immunofluorescence staining of a 9 days old Zebrafish embryo

Specifications

Product Data

• Clone Name: RCK108
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Immunoblotting.
  Immunocytochemistry.
  Flow Cytometry.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Embedded Tissues (Microwave oven based antigen retrieval with destilled water).
  Recommended Dilutions:
  1/100-1/1000 for Immunoblotting and 1/100-1/200 for Flow Cytometry and for Immunohistochemistry with ABC as detection reagent.
• Reactivities: Human, Zebrafish
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cytoskeletal preparation of Human bladder carcinoma cell line T24
• Specificity: Clone RCK108 specifically reacts with Cytokeratin 19, found in most glandular simple type epithelia and most carcinomas.
  Does not react with hepatocytes and hepatocellular carcinoma.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: keratin 19
• Database Link:
  Entrez Gene 3880 Human
  UniProt ID P08727
• Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8.
  The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.
  Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Individual human cytokeratins are numbered 1 to 20.
• Synonyms: Cytokeratin-19, Keratin-19, KRT19, CK19, K19