Granzyme K (GZMK) Mouse Monoclonal Antibody [Clone ID: GM26E7]

CAT#: DM1213

Granzyme K (GZMK) mouse monoclonal antibody, clone GM26E7, Purified

Product Images

Fig.1: FACS analysis of BOSC23 cells using GM-26E7. BOSC23 cells were transiently transfected with an expres-sion vector encoding either Granzyme K (red curve) or anirrelevant protein (control transfectant: black curve). Binding ofGM-26E7 was detected with a PE-conjugated secondaryantibody. A positive signal was obtained only with Granzyme Ktransfected cells.

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  Fig2: BOSC cells were transiently transfected with expression vectors forGranzyme A, B, K, or M. Expression of the constructs was tested with an anti-myc monoclonal antibody (green curves), an irrelevant monoclonal antibodyserved as negative control (black curves). For specificity testing, GM-26E7hybridoma supernatant was tested on all transfectants. A positive signal wasobtained only with Granzyme K transfected cells (red curves).

Specifications

Product Data

• Clone Name: GM26E7
• Applications: ELISA, FC
• Recommended Dilution: Flow cytometry: 1.2 µg/10⁶ cells.
  ELISA: 1/200-1/400.
  ELISA (capture): Clone GM-6C3 as detection antibody.
  Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Genetic immunisation with cDNA encoding human Granzyme K
• Specificity: This antibody reacts to Granzyme K (GZMK).
• Formulation: Phosphate buffered saline, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: granzyme K
• Database Link:
  Entrez Gene 3003 Human
  UniProt ID P49863
• Background: Granzyme K (GrK) belongs to a family of trypsin-like serine proteases localised in the cytotoxic granules of activated T cells and NK cells. It encodes a 28 kDa serine protease whose gene is located on chromosome 5q11-12 close to the granzyme A-encoding gene. Like granzyme A, it has a trypsin-like specifity cleaving at the basic residues arginine and lysine. Granzyme K triggers rapid cell death independently of caspase activation with single-stranded DNA nicks and is primarily expressed in thymus, lung, spleen and peripheral blood leukocytes.
• Synonyms: Granzyme-3, NK-tryptase-2, Fragmentin-3, TRYP2, NK-Tryp-2