CEACAM5 Mouse Monoclonal Antibody [Clone ID: D14HD11]

CAT#: DM1204

CEACAM5 mouse monoclonal antibody, clone D14HD11, Purified

Product Images

Figure 2: Immunofluorescence Microscopy of CHO cells using D14HD11. CHO cells were transfected with an expression vector encoding CEACAM1 (A). Untransfected CHO parental cells served as Negative Control (B). Binding of D14HD11 was visualized with a FITC-conjugated secondary antibody.

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  Figure 3: BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-CEACAM8 or CEACAM19-21. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expressionvector. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1: 4/3/17, CEACAM3, 4: D14HD11, CEACAM5: 26/3/13, CEACAM6: 9A6, CEACAM7: BAC2, CEACAM8: GM-2H6, CEACAM19-21: anti-myc, green curves). An irrelevant monoclonal antibody served as a negative control (black curves). Forspecificity testing, protein G purified D14HD11 was tested on all CEACAM transfectants. A positive signal was obtained with CEA-CAM1, CEACAM3, CEACAM4, CEACAM5 and CEACAM6 expressing cells (red curves).

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  Figure 4: FACS analysis of BOSC23 cells using D14HD11. BOSC23 cells were transiently transfected with anexpression vector encoding either CEACAM1, 3, 4, 5, 6 (red curves) or an irrelevant protein (control transfectant: blackcurves). Binding of D14HD11 was detected with a PE-conjugated secondary antibody. A positive signal wasobtained only with CEACAM1, CEACAM3, CEACAM4, CEACAM5 and CEACAM6 expressing cells.

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  Figure 1: Domain organization of the human CEACAM family. The CEACAM family consists of two subgroups, the CEACAM and the PSG subgroup. CEACAM family members are membrane bound by either via a transmembrane domain or a GPI anchor (green arrow) whereas the PSGs are secreted glycoproteins. N-linked glycosylation sites are shown as drum sticks. The IgV domains are shown. in red, the IgC domains in blue.

Specifications

Product Data

• Clone Name: D14HD11
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Flow Cytometry: 1.2 µg/10⁶ cells.
  The antibody is routinely tested on BOSC23 cells transiently transfected with a CEACAM5 expression vector.
  Cell based ELISA with intakt, transiently transfected cells: 1/200.
  ELISA: 1/200-1/400.
  Western blot: 4 µg/ml.
  Immunofluorescence: 1 µg/10⁶ cells.
  For various aplications see list of references.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Immunisation with extracted protein of CEACAM5
• Specificity: D14HD11 is a broadly reactive antibody which reacts with high affinity (affinity to solid phase bound CEACAM5: 2 x 10e10 L/Mol [6]) with most of the CEACAM molecules (except CEACAM7 and CEACAM8) transiently expressed on the cell surface of transfected BOSC23 cells.
  D14HD11 was included and characterized in the studies from the VIth Leucocyte Typing Workshop. It reacts with the CEACAM family N-domain.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: carcinoembryonic antigen related cell adhesion molecule 5
• Database Link:
  Entrez Gene 1048 Human
  UniProt ID P06731
• Background: CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains (1,2). CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosylphosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumours of epithelial origin is the basis of its wide-spread use as a tumour marker (2). The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumour suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (1).
• Synonyms: CEA, Carcinoembryonic antigen