CEACAM5 Mouse Monoclonal Antibody [Clone ID: D14HD11]

CAT#: DM1204

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CEACAM5 mouse monoclonal antibody, clone D14HD11, Purified



USD 487.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name D14HD11
Applications ELISA, FC, IF, IHC, WB
Recommended Dilution Flow Cytometry: 1.2 µg/106 cells.
The antibody is routinely tested on BOSC23 cells transiently transfected with a CEACAM5 expression vector.
Cell based ELISA with intakt, transiently transfected cells: 1/200.
ELISA: 1/200-1/400.
Western blot: 4 µg/ml.
Immunofluorescence: 1 µg/106 cells.
For various aplications see list of references.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Immunisation with extracted protein of CEACAM5
Specificity D14HD11 is a broadly reactive antibody which reacts with high affinity (affinity to solid phase bound CEACAM5: 2 x 10e10 L/Mol [6]) with most of the CEACAM molecules (except CEACAM7 and CEACAM8) transiently expressed on the cell surface of transfected BOSC23 cells.
D14HD11 was included and characterized in the studies from the VIth Leucocyte Typing Workshop. It reacts with the CEACAM family N-domain.
Formulation PBS, pH 7.2
State: Purified
State: Liquid purified IgG fraction
Concentration lot specific
Purification Affinity Chromatography on Protein G
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens carcinoembryonic antigen related cell adhesion molecule 5 (CEACAM5), transcript variant 1
Background CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains (1,2). CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosylphosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumours of epithelial origin is the basis of its wide-spread use as a tumour marker (2). The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumour suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (1).
Synonyms CEA, Carcinoembryonic antigen
Reference Data
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