HIV-1 (gp41) Mouse Monoclonal Antibody [Clone ID: 417D3.08]

CAT#: DDX1305A546-100

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HIV-1 (gp41) mouse monoclonal antibody, clone 417D3.08


Product Datasheet for 'DDX1305A546-100'


USD 530.00


Availability*
2 Weeks

Size
    • 100 ug


Product images

Specifications

Product Data
Clone Name 417D3.08
Applications ELISA, FC, IF, NEUT
Recommended Dilution DDX1305P-50 / DDX1305P-100 Purified: Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition. 
DDX1305A488-50 / DDX1305A488-100 Alexa- fluor®A488 (on request): Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition.
DDX1305A546-50 / DDX1305A546-100 Alexa- fluor®546 (on request): Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition.
DDX1305A647-50 / DDX1305A647-100 Alexa- fluor®647 (on request): Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition.
DDX1305B-50 / DDX1305B-100 Biotin (on request): Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition. 

Applications tested: Dawood R et al, AIDS. 27(5):717-730, 2013.
Usage recommendation:

*This monoclonal antibody may be used between 5-25 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before using.
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen HR1-PID-HR2-transfected HEK 293 cells (HIV-1, 92BR025, Clade C).
Specificity Conformational epitope formed by aa 531-560 (HR1) and 623-661 (HR2).
Clone 417D3.08 referred to as clone 2 in the article.
Formulation Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
Label: Alexa Fluor 546
Concentration 0.5 mg/ml
Purification QMA Hyper D ion exchange chromatography
Conjugation Alexa Fluor 546
Background HIV-1-gp41 is a subunit of the envelope protein complex of HIV. HIV-1-gp41 is responsible for the fusion between the viral and the cell membranes and thus represents a target for HIV vaccines. Trimeric Env is composed of gp120, which is non-covalently associated with the membrane-anchored fusion protein gp41. HIV-1 gp120 binding to CD4 and co-receptor (CCR5 or CXCR4) induces conformational changes, resulting in gp41 exposure and in the production of fusion-intermediate conformation of gp41 (HR1 and HR2). 3 neutralizing anti-gp41 monoclonal antibodies were selected from mice immunized with 293T cells stably transfected with a construct expressing HR1 plus HR2. (Dawood R et al, AIDS. 27(5):717-730, 2013)
Synonyms HIV1, HIV-I, Human immunodeficiency virus type 1
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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