IL7R alpha (IL7R) Mouse Monoclonal Antibody [Clone ID: R34-34]

CAT#: DDX0700A546-100

IL7R alpha (IL7R) mouse monoclonal antibody, clone R34-34

Product Images

Immunoprecipitation of R34-34 antigen from 125I-surface labelled Pre-Alp cells

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  Facs staining of R34-34-transfected Cos7 cells

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  Facs staining of PBL with R34-34

Specifications

Product Data

• Clone Name: R34-34
• Applications: IF
• Recommended Dilution: DDX0700P-50 / DDX0700P-100 Purified: FACS surface, Neutralization (IL7- binding inhibition), Immunoprecipitation.
  DDX0700A488-50 / DDX0700A488-100 Alexa-fluor®488: Surface Flow cytometry.
  DDX0700A546-50 / DDX0700A546-100 Alexa-fluor®546: IF.
  DDX0700A647-50 / DDX0700A647-100 Alexa-fluor®647: Surface Flow cytometry.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
  
• Reactivities: Canine, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human leukemic pre-B cells.
• Specificity: Human IL7-Rα.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
  Label: Alexa Fluor 546
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 546
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: interleukin 7 receptor
• Database Link:
  Entrez Gene 3575 Human
  UniProt ID P16871
• Background: The IL7-R consists of 2 chains, IL-7Rα known as CD127 and common cytokine receptor γ chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7Rα expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 ; Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54).
• Synonyms: Interleukin-7 receptor subunit alpha, IL-7R-alpha, IL7 receptor alpha