CD283 / TLR3 Mouse Monoclonal Antibody [Clone ID: 1205C5F1 / TLR3.1]

CAT#: DDX0478A647-100

CD283 / TLR3 mouse monoclonal antibody, clone 1205C5F1 / TLR3.1

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Specifications

Product Data

• Clone Name: 1205C5F1 / TLR3.1
• Applications: FC, IHC, IP
• Recommended Dilution: FACS intracellular, ImmunoHistoChemistry paraffin sections, Immunoprecipitation.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: HEK-293 stably transfected with huTLR3+rhu TLR3 ECD (R&D)
• Specificity: Human TLR3.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 647
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 647
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Background: The Toll-like receptor (TLR) family are considered as key for sensing microbial infections and triggering inflammatory and innate responses. 3 novel mAbs were generated against TLR3 that support the emergence of an alternative model for TLR3 biology: TLR3 full-length (TLR3FL, 130KDa) corresponds to the TLR3 present in ER, which in resting cells is steadily converted within the Golgi into fully glycosylated TLR3 (TLR3FL+, 135KDa) and then rapidly cleaved by cathepsins in the endosome/lysosome into 2 long-lived associated fragments (72 KDa and 60KDa). (Toscano F et al, J. Immunol, 2013, 190: 764–773).
• Synonyms: Toll-like receptor 3