Cd207 Rat Monoclonal Antibody [Clone ID: 923B7]

CAT#: DDX0373A546-100

Cd207 rat monoclonal antibody, clone 923B7

Product Images

IHC on human skin cryosections with 923B7-HRP

Specifications

Product Data

• Clone Name: 923B7
• Applications: IF
• Recommended Dilution: DDX0373P-50 / DDX0373P-100 Purified: IHC, Flow cytometry.
  DDX0373A488-50 / DDX0373A488-100 Alexa-fluor®488: IF surface Flow cytometry.
  DDX0373A546-50 / DDX0373A546-100 Alexa-fluor®546: IF.
  DDX0373A647-50 / DDX0373A647-100 Alexa-fluor®647: Flow cytometry.
  DDX0373B-50 / DDX0373B-100 Biotin: Flow cytometry.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
• Reactivities: Human, Mouse, Rat
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human Langerin-transfected eukaryotic cells.
• Specificity: Human Langerin (extracellular epitope).
  Species cross-reactivity: Rat, Mouse.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 546
• Concentration: lot specific
• Purification: Hyper-D ion exchange chromatography
• Conjugation: Alexa Fluor 546
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD207 antigen
• Database Link:
  Entrez Gene 246278 Mouse
  UniProt ID Q8VBX4
• Background: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck’s granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 2002 J Immunol, 168 : 782-792) (Douillard P et al, 2005 J Invest Dermatol, 125: 983–994).
• Synonyms: CLEC4K, Dendritic Cell Marker