Langerin (CD207) Mouse Monoclonal Antibody [Clone ID: DCGM4/122D5.03]

CAT#: DDX0363A546-100

Langerin (CD207) mouse monoclonal antibody, clone DCGM4/122D5.03

Product Images

IHC staining of human skin frozen section with clone 122D5 (DX0363)

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Specifications

Product Data

• Clone Name: DCGM4/122D5.03
• Applications: IF
• Recommended Dilution: DDX0363P-50 DDX0363P-100 Purified: Internalization, Flow cytometry, IHC.
  DDX0363A488-50 DDX0363A488-100 Alexa-fluor®488: Flow cytometry, IF.
  DDX0363A546-50 DDX0363A546-100 Alexa-fluor®546: IF.
  DDX0363A647-50 DDX0363A647-100 Alexa- fluor®647: Flow cytometry.
  DDX0363B-50 DDX0363B-100 Biotin: Western blot.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined y each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
• Reactivities: Canine, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: In vitro-derived human DCs from CD34+ cells in GM-CSF+TNFα
• Specificity: Human Langerin (extracellular epitope in Carbohydrate Recognition domain).
  Species cross-reactivity: Dog.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 546
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 546
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD207 molecule
• Database Link:
  Entrez Gene 50489 Human
  UniProt ID Q9UJ71
• Background: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck’s granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 ; Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)
• Synonyms: CLEC4K, Dendritic Cell Marker