Langerin (CD207) Rat Monoclonal Antibody [Clone ID: 929F3.01]

CAT#: DDX0362A488-100

Langerin (CD207) rat monoclonal antibody, clone 929F3.01

Product Images

IF staining on isolated skin-isolated porcine Langerhans cells

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  on mouse epidermal sheet with 929F3-A488

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  IHC staining of human skin frozen section with clone 929F3 (DX0362)

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  Cells derived from culture of porcine skin stained permeabilized (D) or not (C) with 929F3

Specifications

Product Data

• Clone Name: 929F3.01
• Applications: FC, IF, IHC
• Recommended Dilution: DDX0362P-50 / DDX0362P-100 Purified: IF, IHC formol-paraffin, Flow cytometry.
  DDX0362A488-50 / DDX0362A488-100 Alexa-fluor® 488: IF, IHC , Flow cytometry.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
  
• Reactivities: Human, Mouse, Porcine, Rat
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: human Langerin-transfected eukaryotic cells
• Specificity: Human Langerin.
  Species cross-reactivity: Rat, Mouse, Swine.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 488
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 488
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD207 molecule
• Database Link:
  Entrez Gene 50489 Human
  UniProt ID Q9UJ71
• Background: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck’s granule. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin expression has not been reported outside the DC system. Human Langerin is involved in the processing of mycobacterial glycolipids leading to antigen presentation to CD1a-restricted T cells. Human Langerin binds the gp120 of HIV. (Valladeau J et al, 2002 J. Immunol, 168 : 782-792; Douillard P et al, 2005 J Invest Dermatol, 125: 983–994; Chorro L. et al, J Exp.Med., 2010, 206 (13), 3089-3100).
• Synonyms: CLEC4K, Dendritic Cell Marker